The preparation of biologically active messenger RNA from human postmortem brain tissue

The preparation of biologically active messenger RNA from human postmortem brain tissue

Gilbert JM;Brown BA;Strocchi P;Bird ED;Marotta CA;;
Journal of Neurochemistry 1981 Vol. 36 pp. -
329
jm1981journalthe

Abstract

Messenger RNA (mRNA) was extracted from human postmortem brain tissue by alkaline phenol extraction of polysomes followed by oligo (dT)-cellulose chromatography. The mRNA preparations stimulated protein synthesis in a cell-free system containing wheat germ homogenate. The products of protein synthes …

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