Wooden artifacts are subject to being heavily damaged by the attack of worms which develop in the wood through the eggs deposited by adult pests before their final transformation into flying insects. Among the most dangerous species are xylophagous (wood-boring) insects, whose larvae are responsible for one of the most efficient wood-destroying mechanisms in wooden cultural heritage. Their elimination has always been a huge problem for the conservation of wood. In this work, we present the experimentation of a simple vacuum technique to disinfest wood from the larval Hylotrupes bajolus. We will also introduce the possibility of treating large-sized wooden artifacts by means of a special vacuum chamber.