The vertical rotator is a design based stereological method for estimating object volume using measurements made on co-axial sections. Recently we used the method to estimate eye volume in a number of pathological studies. In the present note we apply an error prediction method for the vertical rotator which has recently been published and analyse the raw data from our earlier study on eye volume in juvenile Dover sole. Application of the new theory allows us to assess the variance contributions due to rotating plane and test line sampling and estimate the underlying biological variability in eye volume. In all cases the coefficient of error introduced by the rotating test was about 10 times greater than due to the test line contribution.